Journal: Cells

Article Title: Cystic Fibrosis Defective Response to Infection Involves Autophagy and Lipid Metabolism

doi: 10.3390/cells9081845

Figure Lengend Snippet: Myriocin (Myr) treatment improved monocyte killing ability. Aspergillus fumigatus conidia were added to patients’ monocytes, untreated or treated with Myr, and cells incubated for 1 h to allow conidia internalization. Non-internalized conidia were then washed, and monocytes were incubated for a further 4 h before undergoing lysis. Live conidia were counted by plating cell lysate on solid medium. To normalize the results, data are expressed as killed conidia/µg protein. Myr partially rescued cystic fibrosis (CF) monocytes’ killing ability (** p < 0.01 Wilcoxon t -test).

Article Snippet: The reference strain A. fumigatus Af293 (ATCC MYA-4609, CBS 101355) was used in the study.

Techniques: Incubation, Lysis

Journal: Pathogens

Article Title: Immunosuppressive Compounds Affect the Fungal Growth and Viability of Defined Aspergillus Species

doi: 10.3390/pathogens8040273

Figure Lengend Snippet: Immunosuppressive compounds at high concentrations reduce the fungal growth of Aspergillus spp. Various concentrations of immunosuppressive compounds were added to resting conidia for 17 hours, and the cell density of A. fumigatus ( A , B ), A. terreus ( C , D ), A. flavus ( E , F ), A. niger ( G , H ) and A. brasiliensis ( I ) mycelium was assessed. Shown are the mean and SEM of at least three independent experiments. Ab— Aspergillus brasiliensis ; Af— A. fumigatus ; Afla— A. flavus ; An— A. niger complex ( A. niger ); At— A. terreus ; mPRED—methylprednisolone; MPA—mycophenolic acid; CsA—cyclosporin A. *: p < 0.05.

Article Snippet: Two different strains each of A. fumigatus (strains AF4215, ATCC MYA 1163 and AF293), A. terreus (clinical isolates T9 and T90, provided by Cornelia Lass-Flörl, Medical University of Innsbruck, Innsbruck, Austria), A. niger complex ( A. niger , clinical isolates BS and 715) and A. flavus (clinical isolates 253 and 467), and one strain of A. brasiliensis (strain ATCC 16404) were grown on Sabouraud glucose agar plates (BD Bioscience, San Jose, CA, USA) at 37 °C for 3 days.

Techniques:

Journal: Pathogens

Article Title: Immunosuppressive Compounds Affect the Fungal Growth and Viability of Defined Aspergillus Species

doi: 10.3390/pathogens8040273

Figure Lengend Snippet: Immunosuppressive compounds reduce the viability of Aspergillus hyphae developing from resting conidia. Measured hyphal damage of A. fumigatus ( A , B ), A. terreus ( C , D ), A. flavus ( E , F ), A. niger ( G , H ) and A. brasiliensis ( I ) when various concentrations of immunosuppressive compounds were added to resting conidia for 17 hours. Shown are the mean and SEM of at least three independent experiments. Ab— Aspergillus brasiliensis ; Af— A. fumigatus ; Afla— A. flavus ; An— A. niger complex ( A. niger ); At— A. terreus ; mPRED—methylprednisolone; MPA—mycophenolic acid; CsA—cyclosporin A. *: p < 0.05.

Article Snippet: Two different strains each of A. fumigatus (strains AF4215, ATCC MYA 1163 and AF293), A. terreus (clinical isolates T9 and T90, provided by Cornelia Lass-Flörl, Medical University of Innsbruck, Innsbruck, Austria), A. niger complex ( A. niger , clinical isolates BS and 715) and A. flavus (clinical isolates 253 and 467), and one strain of A. brasiliensis (strain ATCC 16404) were grown on Sabouraud glucose agar plates (BD Bioscience, San Jose, CA, USA) at 37 °C for 3 days.

Techniques:

Journal: Pathogens

Article Title: Immunosuppressive Compounds Affect the Fungal Growth and Viability of Defined Aspergillus Species

doi: 10.3390/pathogens8040273

Figure Lengend Snippet: Immunosuppressive compounds decrease the viability of fully developed Aspergillus hyphae/mycelium. Measured hyphal damage of A. fumigatus ( A , B ), A. terreus ( C , D ), A. flavus ( E , F ), A. niger ( G , H ) and A. brasiliensis ( I ) when various concentrations of immunosuppressive compounds were added to Aspergillus hyphae for 6 hours. Shown are the mean and SEM of at least three independent experiments. Ab— Aspergillus brasiliensis ; Af— A. fumigatus ; Afla— A. flavus ; An— A. niger complex ( A. niger ); At— A. terreus ; mPRED—methylprednisolone; MPA—mycophenolic acid; CsA—cyclosporin A.

Article Snippet: Two different strains each of A. fumigatus (strains AF4215, ATCC MYA 1163 and AF293), A. terreus (clinical isolates T9 and T90, provided by Cornelia Lass-Flörl, Medical University of Innsbruck, Innsbruck, Austria), A. niger complex ( A. niger , clinical isolates BS and 715) and A. flavus (clinical isolates 253 and 467), and one strain of A. brasiliensis (strain ATCC 16404) were grown on Sabouraud glucose agar plates (BD Bioscience, San Jose, CA, USA) at 37 °C for 3 days.

Techniques: